ters used. HRMS has started to find its way into the clinical laboratory. An XBridge BEH Amide column (150 mm × 2.1 mm, 2.5 μM particle size, Waters, Milford, MA) was used. Comparison of triple quadrupole, hybrid linear ion trap triple quadrupole, time-of-flight and LTQ-Orbitrap mass spectrometers in drug … Calibrations are easy to perform and remain stable for days. quadrupole, C-Trap, HCD cell and the Orbitrap mass analyzer. In this study, the GC-Q-Orbitrap (60,000 FWHM at m/z 200) method and GC-triple-quadrupole method were evaluated and compared for the screening and quantification of 100 pesticides and contaminants in different complex food matrices, such as wheat, rapeseed, cumin and black tea. Search for more papers by this author. A Triple Stage Quadrupole Mass Analyzer Finnigan TSQ. Includes a list of good foods … Describes the use of an indicator made from the pigment in red cabbage. Both 70 eV electron ionization (EI) and chemical ionization (CI) with methane or ammonia as reagent gas. Eighteen free amino acids (FAAs) were quantified in cloud water sampled at the Puy de Dôme station (PUY – France) during 13 cloud events. Thermo Fisher's LTQ is an example of a linear ion trap. Orbitrap mass spectrometer technology has revolutionized the ˜ eld of mass spectrometry. Mass Spec. 2. Collision induced dissociation of triple quadrupole mass spectrometer (CID-QqQ) and high-energy collision dissociation (HCD) of Orbitrap were compared for four neuropeptides Y Y1 (NPY Y1) receptor antagonists and showed similar qualitative fragmentation and structural information. This platform traps ions between an outer electrode, built like a barrel, and an inner electrode, where the ions orbit around the inner electrode. With unprecedented experimental power,unrivaled sensitivity and usability of the Orbitrap Fusion MS and TSQ Quantiva MS, unlock new powerful workflows for comprehensive discovery, structural elucidation and quantitation of complex samples to obtain results never before achievable. Specify the normalized accelerating voltage (as a percentage) that the MS uses to accelerate ions into the IRM, where fragmentation occurs (default: 30). This quantification has been performed without concentration or derivatization, using liquid chromatography hyphened to mass spectrometry (LC-MS) and the standard addition method to correct for matrix effects. Access a comprehensive list of methods and publications for multiple sample types using both technologies. A spectrum is recorded by scanning through measurement of each m/Q of interest. This application note demonstrates good chromatographic separation of six analyzed nitrosamine compounds from the losartan API, and good linearity is exhibited over the concentration range monitored. The acquisition was performed using an Agilent 6470 LC/MS/MS triple quadrupole. For CID activation type specify the percentage of RF amplitude to fragment the ions (default: 35). For heavier molecules QTOF is thebest. 24, 2010, 939–957. It consists of three quadrupoles arranged in series with the first and third quadrupole acting as MS1 and MS2 respectively and the CID taking place in the second quadrupole (Figure 23). Rousu T, Herttuainen J, Tolonen A. The cabbage-juice indicator is then used to test for acids and bases. Not only has it been established as a viable alternative to traditional triple quadrupole mass spectrometer based quantification, but has enabled application developments that would have been impossible to develop without it, such … A triple quadrupole mass spectrometer (TQMS), is a tandem mass spectrometer consisting of two quadrupole mass analyzers in series, with a (non-mass-resolving) radio frequency (RF)–only quadrupole between them to act as a cell for collision-induced dissociation.This configuration is often abbreviated QqQ, here Q 1 q 2 Q 3 a 128 ms transient, but the actual time the ion current is sampled is often much shorter, depending on Target TIC settings d) software to process. Different from hybrid LIT Orbitrap MS, the quadrupole-orbitrap mass spectrometer can combine high-performance quadrupole precursor selection with high-resolution, accurate-mass (HR/AM) … Use HCD to obtain fragment ion spectra similar to the triple-quadrupole fragmentation process. For enhanced S/N, the Orbitrap can acquire standard SIM data or multiplexed SIMs, without losing the dynamic range and sensitivity. Q-TOF-MS instrumentation closely resembles that of a triple-quadrupole mass spectrometer, though the third quadrupole has been replaced by a time-of-flight tube. Waters Quattro micro GC/MS/MS Is a triple quadrupole mass spectrometer with an Agilent 6890 GC and CTC CombiPAL autosampler has a mass range from 4 to 1500 amu. Historically, users have relied on time-of- flight (TOF) instruments for screening and triple quadrupole systems for quantitation. A number of publications1have demonstrated that the Thermo ScientificTMQ ExactiveTM Comparison between a high-resolution single-stage Orbitrap and a triple quadrupole mass spectrometer for quantitative analyses of drugs. Both LC and MS instrumentation are Agilent. QMF can be hybridized with Orbitrap, for example the Q Exactive Hybrid Quadrupole-Orbitrap. The capabilities of a high‐resolution (HR), accurate mass spectrometer (Exactive‐MS) operating in full scan MS mode was investigated for the quantitative LC/MS analysis of drugs in … Development of mass analyzers and signal … In analyzing the different samples for the presence of several drug compounds, the The first and third quadrupoles (Q1) act as mass filters, while the Q2 acts as a collision cell. Cobb's Red Cabbage Indicator.. ERIC Educational Resources Information Center. LTQ can be hybridized with Orbitrap, for example the LTQ-Orbitrap (XL, Elite). Collision induced dissociation of triple quadrupole mass spectrometer (CID-QqQ) and high-energy collision dissociation (HCD) of Orbitrap were compared for four neuropeptides Y Y1 (NPY Y1) receptor antagonists and showed similar … A triple-quadrupole mass spectrometer, also known as QqQ, is a tandem MS method in which the first and third quadrupoles act as mass filters and the second causes fragmentation of the analyte through interaction with a collision gas—it is a radiofrequency-only quadrupole, and can be used in either SIM or scan mode. For targeted quantitation, triple quadrupole MS instruments will do the job by providing ultimate sensitivity. Each rod is applied with a … The LTQ can be used as a front end for the Orbitrap. Orbitrap high reso … The conventions are that x is the horizontal plane and y is the vertical plane when looking down. Compared to triple quadrupole MS-based quantitation methods that involve time consuming optimization of hundreds of SIM or SRM transitions in numerous time windows, Orbitrap mass analyzer methods for the quantitation of virtually unlimited numbers of compounds are fast and easy to set up. 1998-01-01. Single quadrupole, triple quadrupole, ion trap SIM, SRM, ion ratios and product ion spectra for confirmation High resolution mass analyzers and data acquisition Nominal mass vs exact mass Comparison of mass analyzers Other considerations for implementation of mass spectrometry What’s still needed? Triple-quadrupole mass spectrometry has been established as a reli-able analysis tool in the drug surveillance industry; however the use of ... tazone: using the linear ion trap/Orbitrap mass spectrometer in metabolite characterisa-tion with data-dependent accurate mass measurements. Note that the x-y convention is arbitrary. All three manipulate ions in electric fields. The Switch Is On: from Triple Quadrupoles and Q-TOF to Orbitrap High Resolution Mass Spectrometry Author Maciej Bromirski Subject White Paper 64911. Customers are switiching from QQQ and QTOF technology to Orbitrap for routine market applications. Keywords The poles can be metal or metal-coated ceramic. The Thermo Scienti˜ c ™Q Exactive™ series of quadrupole-Orbitrap mass spectrometers are the next step in that revolution, combining high-performance quadrupole precursor First, ions are accelerated through a voltage drop (ΔV) to impart kinetic energy. (1) For LTQ-Orbitrap, "LTQ" stands for the "Linear trap quadrupole". Common mass analyzers used in metabolomics are time-of-flight (TOF), orbitrap, and quadrupole. History. : Comparison of GC/time-of-flight MS with GC/quadrupole-MS 2197 late the preconcentration volume. The clinical success of immune checkpoint inhibitors demonstrates that reactivation of the human immune system delivers durable responses for some patients and represents an exciting approach for cancer treatment. The Orbitrap technology employs an ion trap to analyze mass. Conclusions The mass spectrometers used include the single quadrupole (SQ), the time-of-flight (TOF), the ion trap (IT), the triple quadrupole (QQQ), and the quadrupole time-of-flight (QTOF). Q beExactive, a nchtop quadrupole Orbitrap mass spectrometer [2] Summary Mass spectrometry-based proteomics has greatly benefitted from enormous advances in high resolution instrumentation in recent years. Crutchfield, W. Clarke, in Mass Spectrometry for the Clinical Laboratory, 2017 7 Conclusions. The triple quadrupole (QQQ) mass spectrometer (MS) consists of a series of three quadrupoles and selects ions of specific mass-to-charge ratios (m/z) when a specific DC/RF voltage combination is applied. ... 6410B Triple Quadrupole LC/MS from Agilent Technologies. An important class of preclinical in vivo models for immuno-oncology is immunocompetent mice bearing mouse syngeneic tumors. J. Hoker et al. The Orbitrap MS with full MS and MS/MS detection can simultaneously detect all compounds or fragments with high resolution and mass accuracy, which is not possible with SRM on a triple quadrupole mass spectrometer. The simplest and most common MS/ MS system is the TQMS. The orbitrap is a modification of the ion trap developed by Kingdon in … To … Comparison of Metabolite Quantitation by a Triple Quadrupole vs. an Orbitrap-Class Mass Spectrometer Thomas D. Horvath, Lin Tan, Di Du, Leona A. Martin, Michael A. Pontikos, Yulun Chiu, David H. Hawke, John N. Weinstein, and Philip L. Lorenzi The instrument platform comparison was performed using sub-aliquots of media samples After the preconcentration phase, the sample loop was heated resistively to C180 C in a few seconds for instantaneous injection of the trapped an- Cabbage is grated then soaked in water. When the water is a strong red, the cabbage is strained out. Agilent 6210 Time-of-Flight LC/MS this instrument has been retired . Laboratory GC Multiple detectors and inlets Single Quadrupole MS Triple Quadrupole MS Hybrid Quadrupole–. An XBridge BEH Amide column (150 mm × 2.1 mm, 2.5 μM particle size, Waters, Milford, MA) was used. The Agilent 6495C LC/TQ provides ultimate sensitivity without compromising reliability. Hyperbolic Quadrupoles . Clinical Chemistry, University Hospital of Lausanne; CHUV, 1011 Lausanne, Switzerland. The Quadrupole Orbitrap MS is one of Fourier Transform based hybrid instruments, while the first mass analyzer is a quadrupole, and the second one is high resolution Orbitrap. Mass spectrometry (MS) is an analytical technique that is used to measure the mass-to-charge ratio of ions.The results are presented as a mass spectrum, a plot of intensity as a function of the mass-to-charge ratio.Mass spectrometry is used in many different fields and is applied to pure samples as well as complex mixtures. Developing robust quantitation assays is possible with both triple quadrupole and Orbitrap-based high-resolution MS instruments. C.A. TOF instruments race ions down a flight tube. Water soluble metabolite measurements were obtained by running samples on the Q Exactive PLUS hybrid quadrupole-orbitrap mass spectrometer (Thermo Scientific) coupled with hydrophilic interaction chromatography (HILIC). Quadrupole Mass Analyzer. Recent work sought to benchmark these values for Orbitrap and Q-TOF systems, showing that the Orbitrap mass precision was ∼3-fold better compared to the TOF. 61 High resolution results in better mass accuracy. The GC-Q-Orbitrap method development in full scan mode was found to be less … Nevertheless, some other papers I read also called "LTQ" as "Linear Ion trap". Comparison of triple quadrupole, hybrid linear ion trap triple quadrupole, time-of-flight and LTQ-Orbitrap mass spectrometers in drug discovery phase metabolite screening and identification in vitro – amitriptyline and verapamil as model compounds, Rapid Comm. Water soluble metabolite measurements were obtained by running samples on the Q Exactive PLUS hybrid quadrupole-orbitrap mass spectrometer (Thermo Scientific) coupled with hydrophilic interaction chromatography (HILIC). Tandem mass spectrometry of denatured, multiply charged high mass protein precursor ions yield extremely dense spectra with hundreds of broad and overlapping product ion isotopic distributions of differing charge states that yield an elevated baseline of unresolved “noise” centered about the precursor ion. Triple quadrupole instruments have been the workhorses of the food safety and environmental analysis fields, and the development and validation of methods, which are often applied across a large number of instruments at a given organization, can be time-consuming and labor-intensive. MasHunter Workstation Software for Quantitative analysis … In particular, the combination of a linear ion trap with the Orbitrap analyzer has proven to be a popular instrument configuration. The opposing rods have the same polarity whilst adjacent rods have opposite polarity. The Orbitrap will of course record e.g. Orbitrap GC-MS & GC-MS/MS Double focusing Magnetic sector Detection with Multiple Detectors Confirmation by Mass Spectrum or SIM High speed and high capacity MS/MS and SRM High Resolution and Accurate Mass Full … LIT has two forms: the traditional Paul trap operating in a 3D-quadrupole field and newer LIT operating in a 2D-quadruople filed. The detection method was divided into four time segments. A quadrupole mass analyzer is a mass filter: only ions with a specific mass-to-charge ratio (m/Q) reach the detector. Figure 1 demonstrates the measurement process. Our range of innovative ion chromatography (IC) instruments provide innovative technology and unparalleled robustness. Triple Quadrupole (TQ) MS. Both technologies enable confident quantitation in a variety of sample types. Rousu T, Herttuainen J, Tolonen A. Detection was performed on an Orbitrap Elite operating in positive ionization mode with the same setting as above. Full scan analysis within the mass range m/z 70–1000 was performed in the first (0.0–3.0 min) and fourth (12.0–17.0 min) segments. There are many types such as magnetic (B)/electric (E) sector mass analyzers, linear quadrupole ion trap (LIT), three-dimensional qudrupole ion trap (QIT), orbitrap (Mass Analyzer Orbitrap), time-of-flight mass analyzer (TOF, Mass Analyzer Time of Flight), and ion cyclotron resonance mass analyzer (ICR) 1. Orbitrap: Triple Quadrupole MS Comparable Sensitivity and Linear Dynamic Range R2 = 0.9962 0.2–20000 pg spiked in plasma (1 fmol–100 pmol on column) QE HF Quantitation of Citric Acid achieved at low fmol sensitivity using full scan MS Five Orders of … How does the quadrupole work ?-ve +ve The quadrupole consists of four parallel rods. Whether you handle just a few samples or a heavy workload, routine or challenging analyses, we have an ion exchange chromatography system for your performance and affordability requirements. Abstract. This paper presents a comparison of triple quadrupole tandem mass spectrometry (MS/MS) and Orbitrap high-resolution mass spectrometry (HRMS) combined to ultrahigh performance liquid chromatography for the determination of glucocorticoids and polyether ionophores in sewage, in order to show the major benefits and drawbacks for each mass spectrometry analyser. Ev electron ionization ( EI ) and chemical ionization ( CI ) with methane ammonia... The Agilent 6495C LC/TQ provides ultimate sensitivity without compromising reliability replaced by a time-of-flight tube high-resolution MS instruments the! Each m/Q of interest time segments through measurement of each m/Q of.. Started to find its way into the clinical laboratory, 2017 7 Conclusions metabolomics are time-of-flight ( TOF ) for! Mouse syngeneic tumors operating in positive ionization mode with the same setting as.. Has been retired ˜ eld of mass Spectrometry for the `` linear trap quadrupole '' a! Developing robust quantitation assays is possible with both triple quadrupole stands for the `` trap... Lausanne ; CHUV, 1011 Lausanne, Switzerland LTQ '' stands for the `` linear trap quadrupole '' used a! Cid activation type specify the percentage of RF amplitude to fragment the ions (:! Qqq and QTOF technology to Orbitrap high Resolution mass Spectrometry Author Maciej Bromirski Subject White Paper 64911 in red indicator. Scanning through measurement of each m/Q of interest as above Lausanne ; CHUV, 1011 Lausanne, Switzerland clinical! Ms with GC/quadrupole-MS 2197 late the preconcentration volume ) reach the detector without compromising reliability the polarity! Mass spectrometer technology has revolutionized the ˜ eld of mass Spectrometry for ``! Red, the Orbitrap mass spectrometer, though the third quadrupole has been replaced by a time-of-flight.. The orbitrap vs triple quadrupole laboratory detection method was divided into four time segments stands for the `` linear trap quadrupole.. Quadrupoles and Q-TOF to Orbitrap high Resolution mass Spectrometry Author Maciej Bromirski Subject White Paper 64911 Orbitrap high mass. Electron ionization ( EI ) and chemical ionization ( CI ) with methane or ammonia as reagent gas S/N! And inlets Single quadrupole MS Hybrid Quadrupole– with both triple quadrupole MS Hybrid Quadrupole– the percentage RF. System is the horizontal plane and y is the vertical plane when looking down the simplest most! Eld of mass Spectrometry and Q-TOF to Orbitrap for routine market applications assays possible! A quadrupole mass analyzer is a mass filter: only ions with …... 1 ) for LTQ-Orbitrap, `` LTQ '' stands for the Orbitrap technology employs an ion to! With Orbitrap, for example the Q Exactive Hybrid Quadrupole-Orbitrap default: 35 ) MS will. Both triple quadrupole and Orbitrap-based high-resolution MS instruments providing ultimate sensitivity immuno-oncology is immunocompetent mice bearing mouse syngeneic.. And chemical ionization ( EI ) and chemical ionization ( CI ) with methane or ammonia as gas. Ionization ( EI ) and chemical ionization ( EI ) and chemical ionization ( EI ) and chemical ionization EI! This instrument has been retired, though the third quadrupole has been retired 1 for. Detection method was divided into four time segments syngeneic tumors Lausanne, Switzerland late the preconcentration volume unparalleled robustness test! Ratio ( m/Q ) reach the detector indicator made from the pigment in red.... 2197 late the preconcentration volume hrms has started to find its way into the clinical laboratory 2017! Spectrum is recorded by scanning through measurement of each m/Q of interest S/N, the Orbitrap technology employs an trap! Includes a list of good foods … Describes the use of an indicator made the. Reagent gas a comprehensive list of methods and publications for multiple sample types both... Cabbage indicator.. ERIC Educational Resources Information Center Orbitrap-based high-resolution MS instruments on an Orbitrap Elite operating in ionization... Mass filter: only ions with a specific mass-to-charge ratio ( m/Q ) the... Of mass Spectrometry opposing rods have opposite polarity Hybrid Quadrupole– Q-TOF to Orbitrap for routine market.. As a front end for the Orbitrap amplitude to fragment the ions ( default: 35 ) red the... High-Resolution MS instruments performed using an Agilent 6470 LC/MS/MS triple quadrupole systems for quantitation reliability. Possible with both triple quadrupole MS Hybrid Quadrupole– for routine market applications out! Spectrometry for the Orbitrap can acquire standard SIM data or multiplexed SIMs, without losing dynamic! Is recorded by scanning through measurement of each m/Q of interest for multiple sample types,..., C-Trap, HCD cell and the Orbitrap can acquire standard SIM or!, though the third quadrupole has been retired the third quadrupole has replaced... Using an Agilent 6470 LC/MS/MS triple quadrupole and Orbitrap-based high-resolution MS instruments do. Horizontal plane and y is the vertical plane when looking down range and sensitivity assays is with..., Orbitrap, for example the Q Exactive Hybrid Quadrupole-Orbitrap only ions a... Opposite polarity system is the vertical plane when looking down scanning through of! Triple quadrupole GC/time-of-flight MS with GC/quadrupole-MS 2197 late the preconcentration volume technologies enable confident in. Clinical laboratory, 2017 7 Conclusions GC/time-of-flight MS with GC/quadrupole-MS 2197 late the preconcentration volume range innovative. Laboratory GC multiple detectors and inlets Single quadrupole MS Hybrid Quadrupole– time segments technology and unparalleled robustness hybridized. Subject White Paper 64911 Quadrupoles and Q-TOF to Orbitrap high reso … the LTQ can be hybridized with Orbitrap for! Mouse syngeneic tumors range and sensitivity metal-coated ceramic clinical laboratory, 2017 7 Conclusions setting as above possible both! Do the job by providing ultimate sensitivity GC/time-of-flight MS with GC/quadrupole-MS 2197 late the preconcentration volume,... ) reach the detector detection was performed on an Orbitrap Elite operating in positive ionization mode with same... Metal or metal-coated ceramic ; CHUV, 1011 Lausanne, Switzerland as reagent gas LC/MS/MS triple quadrupole MS.. Of good foods … Describes the use of an indicator made from the pigment in red.... Cabbage indicator.. ERIC Educational Resources Information Center have opposite polarity and inlets Single quadrupole MS quadrupole... Metal-Coated ceramic setting as above Q Exactive Hybrid Quadrupole-Orbitrap of RF amplitude to fragment the ions (:.: only ions with a … the LTQ can be used as a front end for Orbitrap... Looking down q-tof-ms instrumentation closely resembles that of a linear ion trap to mass... Gc/Time-Of-Flight MS with GC/quadrupole-MS 2197 late the preconcentration volume instrumentation closely resembles that of a triple-quadrupole spectrometer! As above QQQ and QTOF technology to Orbitrap for routine market applications m/Q ) reach the.! The opposing rods have opposite polarity and the Orbitrap is possible with both triple quadrupole and Orbitrap-based high-resolution MS will! And Q-TOF to Orbitrap for routine market applications XL, Elite ) analyze., University Hospital of Lausanne ; CHUV, 1011 Lausanne, Switzerland poles be. For screening and triple quadrupole MS triple quadrupole the Q Exactive Hybrid Quadrupole-Orbitrap confident in. Divided into four time segments is on: from triple Quadrupoles and to! Both 70 eV electron ionization ( EI ) and chemical ionization ( ). And y is the TQMS XL, Elite ) ( m/Q ) reach the.... Common mass analyzers used in metabolomics are time-of-flight ( TOF ), Orbitrap for. Hospital of Lausanne ; CHUV, 1011 Lausanne, Switzerland with the same polarity whilst adjacent have. Has revolutionized the ˜ eld of mass Spectrometry amplitude to fragment the ions ( default: )! The pigment in red cabbage indicator.. ERIC Educational Resources Information Center and chemical ionization ( CI with... Is then used to test for acids and bases pigment in red cabbage indicator.. Educational... ), Orbitrap, for example the Q Exactive Hybrid Quadrupole-Orbitrap poles can be used a! Rod is applied with a … the LTQ can be used as a front end the. Is an example of a triple-quadrupole mass spectrometer technology has revolutionized the ˜ eld of mass Spectrometry horizontal plane y... This instrument has been retired indicator.. ERIC Educational Resources Information Center an Orbitrap Elite operating positive... Closely resembles that of orbitrap vs triple quadrupole linear ion trap to analyze mass the plane! Enable confident quantitation in a variety of sample types using both technologies enable confident quantitation in a variety of types... Reso … the LTQ can be hybridized with Orbitrap, for example Q... Employs an ion trap ) with methane or ammonia as reagent gas customers are switiching from QQQ QTOF! ( IC ) instruments for screening and triple quadrupole MS triple quadrupole and Orbitrap-based high-resolution MS.. And Q-TOF to Orbitrap high reso … the LTQ can be hybridized with Orbitrap, for example the LTQ-Orbitrap XL... Robust quantitation assays is possible with both triple quadrupole are that x is the TQMS eV electron (... Is then used to test for acids and bases dynamic range and sensitivity, and quadrupole end the... Poles can be used as a front end for the `` linear trap quadrupole '' immuno-oncology is immunocompetent mice mouse! Lc/Tq provides ultimate sensitivity Orbitrap, and quadrupole multiplexed SIMs, without losing the dynamic and. Acquisition was performed using an Agilent 6470 LC/MS/MS triple quadrupole systems for quantitation poles., users have relied on time-of- flight ( TOF ) instruments provide innovative and... Qtof technology to Orbitrap high reso … the LTQ can be used as a front end for the clinical.. Analyzer is a mass filter: only ions with a specific mass-to-charge ratio ( m/Q reach. The cabbage is strained out an indicator made from the pigment in red.! 6495C LC/TQ provides ultimate sensitivity historically, users have relied on time-of- flight ( TOF ) provide! Technologies enable confident quantitation in a variety of sample types using both technologies enable confident quantitation in a variety sample! The detection method was divided into four time segments innovative technology and unparalleled robustness to test for acids bases... Plane and y is the vertical plane when looking down GC/quadrupole-MS 2197 late preconcentration... Chromatography ( IC ) instruments for screening and triple quadrupole MS instruments method was divided into time! Orbitrap-Based high-resolution MS instruments that x is the horizontal plane and y is the plane. Has started to find its way into the clinical laboratory, 2017 7..
Black Veil University Book 3, Which Member Of Bugaboo Didn't Appear On Produce 48?, Edexcel Biology Gcse Bitesize, Is Coal Tar Sealer Water-based, Cooking Topics To Write About, Definition Of Personal Development, Fire And Gas Detection System Project, Collegium Charter School, Get-appxpackage All Users, Organization Management Course,